The Center for Engineering in Medicine & Surgery at Massachusetts General Hospital merges the principles of engineering design and analysis with medicine, molecular biology and biochemistry in order to find new solutions that push the envelope of healthcare technology and the practice of medicine. It is engaged in a variety of research thrusts with a major focus on Biopreservation, Organ Reengineering, Tissue Engineering and Regenerative Medicine. The Biopreservation research focuses on developing novel preservation technologies to support the fundamental and practical issues of cryopreservation by vitrification, supercooling to subzero temperature and partial freezing of cells, tissues, organs and organisms. Organ Reengineering aims at developing applications to increase the viability of transplantable organs and ultimately eliminate the donor organ shortage. Its major emphasis is on an organ revitalization platform based on machine perfusion. The Center also explores new diagnostic tools to assess preservation modality, damage and viability of all biological systems.